Human Phospho-MAPK Array Kit

• Human Phospho-MAPK Array Kit Ordering Information

Introduction

The Human Phospho-Mitogen-activated Protein Kinase (MAPK) Antibody Array (Catalog # ARY002B) is an economical tool used to simultaneously detect the relative phosphorylation of 24 kinases without performing numerous immunoprecipitation and/or Western blots. Kinases are captured by 26 different antibodies spotted in duplicate on a nitrocellulose membrane. Levels of phosphorylated protein are then assessed using phospho-specific antibodies and chemiluminescent detection.

• Product Insert (PDF, 950K)
• Troubleshooting Guide
• Assays for analytes represented in the Human Phospho-MAPK Array Kit
• Western blotting antibodies for analytes related to the Human Phospho-MAPK Array Kit

General Assay Principle

Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell lysate samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any protein/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of cytokine bound. Chemiluminescence is detected in the same manner as a Western blot.

Kit Contents

Each Human MAPK Antibody Array Kit contains most of the necessary components for a ready-to-run assay without the need for specialized equipment.*

• 4-Array Membranes
• 4-Well Multi-dish
• Lysis Buffer
• Array Buffers
• Wash Buffer
• Detection Antibody Cocktail
• Streptavidin-HRP
• Chemiluminescent Detection Reagents
• Transparency Overlay Template
• Detailed product datasheet with protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/ Other Supplies Required sections of the product datasheet.